|
KMID : 0358419750180010001
|
|
Korean Journal of Obstetrics and Gynecology
1975 Volume.18 No. 1 p.1 ~ p.9
|
|
|
A Comparative Study on the Membraneous Protein of Meternal and Neonatal Erythrocytes
|
|
權純旭/Kwon, Sun Uk
李元起/曺龍鎬/Lee, won Ki/Cho, Yong Ho
|
|
|
Abstract
|
|
|
Both maternal and neonatal ghosts of erythrocytes were obtained by differential centrifugation and The protein fractions constituent of erythrocyte membranes were compared. When the membraneous proteins were subjected to 5.6% polyacrylamide gel electrophoresiss upon the disolution into sodium , dodecylsulfatemercaptoethanol mixture significant differences between the maternal and neonatal erythrocytes were observed as follows.
1. The optimum wave length for Amido Black 10 B dye solution was found to be
620 nm.
2. The membraneous protein of erythrocyte was fractionated into 5 bands namely, band I II. N? V and V in the case of maternal blood in good contrast to 7 bands for the neonatal blood namely, band I, 1I, 19, N, V, VI, -and W.
3. Compared to- the fraction bands of neonatal membraneous protein those of maternal protein were 2 _bands less is e., band III and MI
4: In the fractions of maternal membranous protein band , I. was quantitatively the most abundant (37.4%) followed by bands N, 11, V and lI´ in decreasing order.
5. ´~In the-cases of neonatal erythrocytes the membraneous protein fractionated, into band Vl constitued 2.2.3% followed by bands l , IV, WW, ll and 11 in quantitatively decreasing order.
6. Band 11 and band V were found to be identical for both maternal and neonatal
erythrocytes quantitatively and in terms of electrophoretical mobility.
7. A relatively rapid mobility of band V3 and Ill in the neonatal case is indicated that
constituent amino acids of membraneous protein are acidic amino acids with more carboxylic groups.
8. Serum protein was fractionated into 11 regions in the cases of both maternal and neonatal bloods.
In view of the above observation it is concluded that there is a definite difference between membranous proteins of maternal and neonatal erythrocytes and it is reasoned
to be due to the independent erythropoietic sites for two individuals.
|
|
|
KEYWORD
|
|
|
|
|
|
FullTexts / Linksout information
|
|
 
|
|
|
Listed journal information
|
|
  
|
|